development of a double glass mounting method using formaldehyde alcohol azocarmine lactophenol (faal) and its evaluation for permanent mounting of small nema¬todes

background: : permanent slide preparation of nematodes especially small ones is time consuming, difficult and they become scarious margins. regarding this prob­lem, a modified double glass mounting method was developed and com­pared with classic method. methods: a total of 209 nematode samples from human and animal origin were fixed and stained with formaldehyde alcohol azocarmine lactophenol (faal) followed by double glass mounting and classic dehydration method us­ing canada balsam as their mounting media. the slides were evaluated in differ­ent dates and times, more than four years. different photos were made with differ­ent magnification during the evaluation time. results: the double glass mounting method was stable during this time and compa­rable with classic method. there were no changes in morphologic struc­tures of nematodes using double glass mounting method with well-defined and clear differentiation between different organs of nematodes in this method. conclusion: using this method is cost effective and fast for mounting of small nematodes comparing to classic method.